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Hydrogen production by bacteria of genus Clostridium
Sedláček, Zbyněk ; Turková, Kristýna (referee) ; Rittich, Bohuslav (advisor)
The bachelor thesis deals hydrogen production by bacteria of the genus Clostridium. Thesis gives an overview about hydrogen production mentioned microoganism, characterized further biotechnological methods and show examples of industrial production of hydrogen. Also there are describes the quantitative characteristic and metabolism of bacteria genus Clostridium and examples of usable substrates. Polymerase chain reaction (PCR) is used to the detection and identification by species and specific primers. DNA isolated by fenol extraction from bacterial culture Clostridium tyrobutyricum DSM 2637 was amplified using genus-specific PCR to form PCR products size 619 bp.
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Molecular characterization of selected clostridial strains isolated from cheeses
Chroboková, Maria ; Kvasničková, Eva (referee) ; Rittich, Bohuslav (advisor)
The study was focused on molecular characterization of 42 clostridial strains. DNA was isolated by fenol-chloroform extraction procedure and precipitated with ethanol. After DNA isolation, PCR amplifications with specific primer sets were used for genus and species identification. Finally 19 strains were clasified as Clostridium tyrobutyricum and 3 strains were clasified as Clostridium butyricum. Presence of hydrogenase gene hydA was tested by PCR amplification using specific primer set HGf and HGr. Presence of hydrogenase gene was detected within 21 strains. (GTG)5 primer (rep-PCR) and Pr1 and Pr6 primers (RAPD) were used for differentiation of clostridial strains. Next, the cultivation of Clostridium tyrobutyricum S5 was studied under different conditions. The cultivation was carried out in liquid Reinforced Clostridial Medium (RCM) with lactose and cheese whey instead of glucose under anaerobe conditions. Growth was observed at laboratory temperature (20 to 23 °C) and at 37 °C, pH values ranging from 4.0 to 8.0 with 0.5 unit.
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Signaling Pathway for Butanol Production in Solventogenic Clostridium Bacteria
Musilová, Jana ; Škutková, Helena (referee) ; Sedlář, Karel (advisor)
Diplomová práce se zabývá studiem signální dráhy produkce butanolu bakterií rodu Clostridium. V první části pojednává o modelování signálních drah pomocí metod systémové biologie. Navazuje popisem zisku dat pro tvorbu a úpravu modelů signálních drah s hlavním zaměřením na techniky pro zjištění genové exprese, produkce a fenotypu. Třetí sekcí je získání základního modelu signální dráhy zapojené do produkce butanolu u solventogenních klostridií. Posledním bodem a zároveň hlavním cílem je vytvoření dynamického modelu signální dráhy produkce butanolu kmene Clostridium beijerinckii NRRL B-598, jeho vyhodnocení pomocí statické a dynamické analýzy a srovnání s biologickými daty.
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Signaling Pathway For Butanol Production In Clostridium Beijerinckii Nrrl B-598
Musilová, Jana
In this study, we bring the first dynamic model of signaling pathway for butanol production in sporulating, solvent-producing bacterium, Clostridium beijerinckii NRRL B-598. The model allows to study functions of individual genes involved in the production of solvents and acids. We used an open platform Cell Collective for designing the model and stored it under the name ‘Signaling Pathway for Butanol Production in Clostridium beijerinckii NRRL B-598’ version 1.0 (https://research.cellcollective.org/?dashboard=true#36604:1/signaling-pathway-for-butanol-production-inclostridium-beijerinckii-nrrl-b598/10) in publicly available repository of Cell Collective.
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Signaling Pathway for Butanol Production in Solventogenic Clostridium Bacteria
Musilová, Jana ; Škutková, Helena (referee) ; Sedlář, Karel (advisor)
Diplomová práce se zabývá studiem signální dráhy produkce butanolu bakterií rodu Clostridium. V první části pojednává o modelování signálních drah pomocí metod systémové biologie. Navazuje popisem zisku dat pro tvorbu a úpravu modelů signálních drah s hlavním zaměřením na techniky pro zjištění genové exprese, produkce a fenotypu. Třetí sekcí je získání základního modelu signální dráhy zapojené do produkce butanolu u solventogenních klostridií. Posledním bodem a zároveň hlavním cílem je vytvoření dynamického modelu signální dráhy produkce butanolu kmene Clostridium beijerinckii NRRL B-598, jeho vyhodnocení pomocí statické a dynamické analýzy a srovnání s biologickými daty.
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Molecular characterization of selected clostridial strains isolated from cheeses
Chroboková, Maria ; Kvasničková, Eva (referee) ; Rittich, Bohuslav (advisor)
The study was focused on molecular characterization of 42 clostridial strains. DNA was isolated by fenol-chloroform extraction procedure and precipitated with ethanol. After DNA isolation, PCR amplifications with specific primer sets were used for genus and species identification. Finally 19 strains were clasified as Clostridium tyrobutyricum and 3 strains were clasified as Clostridium butyricum. Presence of hydrogenase gene hydA was tested by PCR amplification using specific primer set HGf and HGr. Presence of hydrogenase gene was detected within 21 strains. (GTG)5 primer (rep-PCR) and Pr1 and Pr6 primers (RAPD) were used for differentiation of clostridial strains. Next, the cultivation of Clostridium tyrobutyricum S5 was studied under different conditions. The cultivation was carried out in liquid Reinforced Clostridial Medium (RCM) with lactose and cheese whey instead of glucose under anaerobe conditions. Growth was observed at laboratory temperature (20 to 23 °C) and at 37 °C, pH values ranging from 4.0 to 8.0 with 0.5 unit.
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Hydrogen production by bacteria of genus Clostridium
Sedláček, Zbyněk ; Turková, Kristýna (referee) ; Rittich, Bohuslav (advisor)
The bachelor thesis deals hydrogen production by bacteria of the genus Clostridium. Thesis gives an overview about hydrogen production mentioned microoganism, characterized further biotechnological methods and show examples of industrial production of hydrogen. Also there are describes the quantitative characteristic and metabolism of bacteria genus Clostridium and examples of usable substrates. Polymerase chain reaction (PCR) is used to the detection and identification by species and specific primers. DNA isolated by fenol extraction from bacterial culture Clostridium tyrobutyricum DSM 2637 was amplified using genus-specific PCR to form PCR products size 619 bp.
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